Em cũng đọc được trên một tài liệu : họ đồng nhất embryonic germ cell và embryonic stem cell làm một
ESCs và EGCs là hai khái niệm để chỉ hai loại tế bào khác nhau. Hiểu nôm na thì ESCs là tổ tiên của tất cả các tế bào trong cơ thể còn EGCs là cha ông của tinh trùng và trứng. Như vậy cũng có nghĩa EGCs bắt nguồn từ ESCs.
Mình đã đọc một bài về nuôi cấy EGCs của dê Guanzhong. Bạn có thể xem trích dẫn dưới đây:
"2.2. Isolation and culture of goat embryonic germ
To isolate Primordial Germ Cells, nine fetuses were collected fromseven white Guanzhong dairy goat fetuses at 28–42 days of pregnancy . The gonadal ridge tissue was
removed, washed three times with PBS plus 0.02%
ethylenediaminetetraacetic acid (EDTA), dissected
manually, and incubated for 30 min at 38.5 8C in a cell
dissociation solution containing 0.25% collagenase IV.
The cell suspension was filtered through sterile gauze
(100 mesh, 149 micrometer ) and washed in PBS once, then
pelleted by centrifugation at 1000 xg for 5 min. The
suspension of cell mixture of gonadal tissue was co-cultured
with goat embryonic fibroblasts (GEF) that had
been inactivated with mitomycin C treatment on gelatincoated culture dishes. After 10–12 days of growth, EG-like cell colonies with 100–200 cells were formed and
then subcultured by picking up individual colonies and
seeding on 35 mm culture dish; this subculture was
considered the first passage of EG cells. Although goat
EG cells can grow well with both GEF and mouse
embryonic fibroblast (MEF), we routinely used MEF as
the feeder cells to culture goat EG cells, since it was
easier to use. For the immunohistochemistry assay, MEF
and EG cells were cultured on cover slides that were laid
on a culture plate. The culture medium was DMEM
supplemented with 15% KSR, 1000 IU/mL LIF, 10 ng/
mL bFGF, 10 ng/mL SCF, 0.1 mM nonessential amino
acids, 0.1 mM b-mercaptoethanol, 2 mM L-glutamine,
100 IU/mL penicillin, and 0.1 mg/mL streptomycin. The
above three factors, LIF, bFGF and SCF, were always
added in media as supplements, even when typical EG
colonies were formed.
The isolated EG cells were cryopreserved in liquid
nitrogen by a method similar to that used for MEF cells.
Briefly, 5 x 105 cells collected from cultural plates
were pelleted in a 10 mL centrifuge tube, and resuspended in 1 mL of cryo-storage solution containing
DMEM with 20% fetal bovine serum and 10%
dimethyl sulfoxide. Cells were then transferred to a
storage tube (2 mL) and stored in liquid nitrogen. To
reuse the cryopreserved cells, a tube of cells was
removed from liquid nitrogen and immediately transferred
to a 37 oC water bath to incubate for 1–2 min.
The thawed cells were subsequently cultured in the
60 mm plate with MEF feeder cells. "