Nguyễn Trọng Tường
Senior Member
The T. reesei cbh2 gene was amplified from first strand cDNA prepared
from T. reesei with primers TRECBH2-L and TRECBH2-R. Sequence analysis
showed that the cloned cbh2 contained three introns. Overlap PCR with
primers CBH2XS-int1, CBH2-int2, CBH2-int3 and TRECBH2-R were used to
remove the native secretion signal and all of the introns.
Em không rõ tại sao đã khuyếch đại cDNA sao lại còn intron để overlap PCR là sao.Giúp em với....Em không hiểu rõ cơ chế lắm..
from T. reesei with primers TRECBH2-L and TRECBH2-R. Sequence analysis
showed that the cloned cbh2 contained three introns. Overlap PCR with
primers CBH2XS-int1, CBH2-int2, CBH2-int3 and TRECBH2-R were used to
remove the native secretion signal and all of the introns.
Em không rõ tại sao đã khuyếch đại cDNA sao lại còn intron để overlap PCR là sao.Giúp em với....Em không hiểu rõ cơ chế lắm..