Hồ Xuân Hùng
Senior Member
Các bác ơi, em đang cần 1 cuốn Manipulating the Mouse Embryo, của Andras Nagy. ko biết có ai có thì share em với. chả là em đang phải làm về mouse oogenesis. thanks
Nhờ lấy giúp bài báo khoa học
- Thread starter Nguyễn Thành Đạt
- Start date
Hà Thị Minh Thi
Senior Member
Nhờ mọi người lấy giúp mình bài báo này với. Cám ơn nhiều nha.
Nature Clinical Practice Gastroenterology & Hepatology</SPAN> (2004) 1, 9
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fficeffice" /><o></o>
Early detection in gastric cancer
Nature Clinical Practice Gastroenterology & Hepatology</SPAN> (2004) 1, 9
<ABBR title="Digital Object Identifier" minmax_bound="true">doi</ABBR minmax_bound="true">:10.1038/ncpgasthep0023 <?xml:namespace prefix = o ns = "urn:schemas-microsoft-com
fficeffice" /><o></o>Early detection in gastric cancer
Nguyễn Phước Hùng
Senior Member
Các bác hảo tâm cho em xin một bài protocol trên spingerlink với.
Clustal V: Multiple Alignment of DNA and Protein Sequences
http://www.springerprotocols.com/Abstract/doi/10.1385/0-89603-276-0:307
Thanks các bác nhiều!
Clustal V: Multiple Alignment of DNA and Protein Sequences
http://www.springerprotocols.com/Abstract/doi/10.1385/0-89603-276-0:307
Thanks các bác nhiều!
Nguyễn Duy Hưng
Senior Member
@Minh Thi: bài báo của bạn trên NATURE CLINICAL PRACTICE GASTROENTEROLOGY & HEPATOLOGY chỉ có nhiêu đó thôi
Early detection in gastric cancer
Successful treatment in gastric cancer is limited by the late detection of tumors. A recent paper by Koike and colleagues compares two PCR-based screening methods for early detection.
Preoperative blood samples were obtained from 41 gastric cancer patients and 10 healthy volunteers. Samples were subjected to two assays: CEA-specific nested RT–PCR, in which a positive result indicates that circulating tumor cells are present in the original sample; and the MSP assay, which differentiates between methylated and unmethylated promoter regions of the p16, E-cadherin and RARβ genes. Hypermethylation is a marker of downregulation or silencing of tumor suppressor genes, and hence indicates that tumor DNA fragments are present.
The CEA-specific RT–PCR assay gave a positive result in 10 (24%) patients and the detection rate was higher in stage III or IV disease than in stages I or II (P<0.01). The MSP assay detected aberrant methylation of p16 in 9 (22%) patients, E-cadherin in 9 (22%) patients and RARβ in 6 (15%) patients. Overall, 18 (44%) patients showed aberrant methylation of the promoter region of at least one of the genes tested, and a positive result correlated with venous invasion (P<0.05).
While the overall detection rate of the MSP assay was higher than that for CEAspecific RT–PCR, Koike et al. suggest that both assays will be useful for identifying patients requiring more intensive screening and treatment.
Original article Koike H et al. (2004) Comparison of methylation-specific polymerase chain reaction (MSP) with reverse transcriptase-polymerase chain reaction (RT-PCR) in peripheral blood of gastric cancer patients. J Surg Oncol 87: 182–186
@Hùng, bài của bạn đây
http://ifile.it/6i2le3g
Early detection in gastric cancer
Successful treatment in gastric cancer is limited by the late detection of tumors. A recent paper by Koike and colleagues compares two PCR-based screening methods for early detection.
Preoperative blood samples were obtained from 41 gastric cancer patients and 10 healthy volunteers. Samples were subjected to two assays: CEA-specific nested RT–PCR, in which a positive result indicates that circulating tumor cells are present in the original sample; and the MSP assay, which differentiates between methylated and unmethylated promoter regions of the p16, E-cadherin and RARβ genes. Hypermethylation is a marker of downregulation or silencing of tumor suppressor genes, and hence indicates that tumor DNA fragments are present.
The CEA-specific RT–PCR assay gave a positive result in 10 (24%) patients and the detection rate was higher in stage III or IV disease than in stages I or II (P<0.01). The MSP assay detected aberrant methylation of p16 in 9 (22%) patients, E-cadherin in 9 (22%) patients and RARβ in 6 (15%) patients. Overall, 18 (44%) patients showed aberrant methylation of the promoter region of at least one of the genes tested, and a positive result correlated with venous invasion (P<0.05).
While the overall detection rate of the MSP assay was higher than that for CEAspecific RT–PCR, Koike et al. suggest that both assays will be useful for identifying patients requiring more intensive screening and treatment.
Original article Koike H et al. (2004) Comparison of methylation-specific polymerase chain reaction (MSP) with reverse transcriptase-polymerase chain reaction (RT-PCR) in peripheral blood of gastric cancer patients. J Surg Oncol 87: 182–186
@Hùng, bài của bạn đây
http://ifile.it/6i2le3g
Hồ Xuân Hùng
Senior Member
<meta http-equiv="Content-Type" content="text/html; charset=utf-8"><meta name="ProgId" content="Word.Document"><meta name="Generator" content="Microsoft Word 11"><meta name="Originator" content="Microsoft Word 11"><link rel="File-List" href="file:///C:%5CDOCUME%7E1%5CHungK9%5CLOCALS%7E1%5CTemp%5Cmsohtml1%5C01%5Cclip_filelist.xml"><!--[if gte mso 9]><xml> <w:WordDocument> <w:View>Normal</w:View> <w:Zoom>0</w:Zoom> <wunctuationKerning/> <w:ValidateAgainstSchemas/> <w:SaveIfXMLInvalid>false</w:SaveIfXMLInvalid> <w:IgnoreMixedContent>false</w:IgnoreMixedContent> <w:AlwaysShowPlaceholderText>false</w:AlwaysShowPlaceholderText> <w:Compatibility> <w:BreakWrappedTables/> <w:SnapToGridInCell/> <w:WrapTextWithPunct/> <w:UseAsianBreakRules/> <wontGrowAutofit/> </w:Compatibility> <w:BrowserLevel>MicrosoftInternetExplorer4</w:BrowserLevel> </w:WordDocument> </xml><![endif]--><!--[if gte mso 9]><xml> <w:LatentStyles DefLockedState="false" LatentStyleCount="156"> </w:LatentStyles> </xml><![endif]--><style> <!-- /* Style Definitions */ p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-parent:""; margin:0in; margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";} h2 {mso-margin-top-alt:auto; margin-right:0in; mso-margin-bottom-alt:auto; margin-left:0in; mso-pagination:widow-orphan; mso-outline-level:2; font-size:18.0pt; font-family:"Times New Roman";} a:link, span.MsoHyperlink {color:blue; text-decoration:underline; text-underline:single;} a:visited, span.MsoHyperlinkFollowed {colorurple; text-decoration:underline; text-underline:single;} @page Section1 {size:8.5in 11.0in; margin:1.0in 1.25in 1.0in 1.25in; mso-header-margin:.5in; mso-footer-margin:.5in; mso-paper-source:0;} div.Section1 {page:Section1;} --> </style><!--[if gte mso 10]> <style> /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} </style> <![endif]--> Nhờ mọi người lấy giúp em bài này
Expression and purification of active form of HIV-1 protease from E.coli. http://www.ncbi.nlm.nih.gov/pubmed/7627139?ordinalpos=2&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_DefaultReportPanel.Pubmed_RVDocSum
Thanks.
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Thanks.
Lấy giùm tôi bài trên Nature Protocol với. Xin cám ơn nhiều
http://www.nature.com/nprot/journal/v3/n11/pdf/nprot.2008.175.pdf
http://www.nature.com/nprot/journal/v3/n11/pdf/nprot.2008.175.pdf
Lê Đoàn Thanh Lâm
Senior Member
Nhờ mọi người lấy giúp mấy bài sau :
<?xml:namespace prefix = o ns = "urn:schemas-microsoft-comfficeffice" /><o></o>
1, Atomic force microscopy of microbial cells: Application to nanomechanical properties, surface forces and molecular recognition forces
Colloids and Surfaces B: Biointerfaces, Volume 54, Issue 1, 15 January 2007, Pages 10-19
<o></o>
<o></o>
2, Direct measurement of hydrophobic forces on cell surfaces using AFM. Langmuir.</SPAN> 2007 Nov 20;23(24):11977-9. Epub 2007 Oct 17<o></o>
<o></o>
3, Chemical force microscopy of single live cells. Nano Lett.</SPAN> 2007 Oct;7(10):3026-30. Epub 2007 Sep 13.<o></o>
<o></o>
4, Combined use of atomic force microscopy, X-ray photoelectron spectroscopy, and secondary ion mass spectrometry for cell surface analysis. Langmuir. 2008 Apr 1;24(7):2955-9. Epub 2008 Feb 1.<o></o></SPAN>
<o></o>
5, Atomic force microscopy and chemical force microscopy of microbial cells. Nat Protoc. 2008;3(7):1132-8. http://www.nature.com/nprot/journal/v3/n7/abs/nprot.2008.101.html <o></o></SPAN>
<o>Thanks a lot!
</o>
<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com
fficeffice" /><o></o>1, Atomic force microscopy of microbial cells: Application to nanomechanical properties, surface forces and molecular recognition forces
Colloids and Surfaces B: Biointerfaces, Volume 54, Issue 1, 15 January 2007, Pages 10-19
<o
></o> <o
></o>2, Direct measurement of hydrophobic forces on cell surfaces using AFM. Langmuir.</SPAN> 2007 Nov 20;23(24):11977-9. Epub 2007 Oct 17<o
></o><o
></o>3, Chemical force microscopy of single live cells. Nano Lett.</SPAN> 2007 Oct;7(10):3026-30. Epub 2007 Sep 13.<o
></o><o
></o>4, Combined use of atomic force microscopy, X-ray photoelectron spectroscopy, and secondary ion mass spectrometry for cell surface analysis. Langmuir. 2008 Apr 1;24(7):2955-9. Epub 2008 Feb 1.<o
></o></SPAN><o
></o>5, Atomic force microscopy and chemical force microscopy of microbial cells. Nat Protoc. 2008;3(7):1132-8. http://www.nature.com/nprot/journal/v3/n7/abs/nprot.2008.101.html <o
></o></SPAN><o
>Thanks a lot!</o
>
Nguyễn Phước Hùng
Senior Member
Nhờ mọi người lấy giúp mấy bài sau :
<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com
1, Atomic force microscopy of microbial cells: Application to nanomechanical properties, surface forces and molecular recognition forces
Colloids and Surfaces B: Biointerfaces, Volume 54, Issue 1, 15 January 2007, Pages 10-19
<o
<o
2, Direct measurement of hydrophobic forces on cell surfaces using AFM. Langmuir.</SPAN> 2007 Nov 20;23(24):11977-9. Epub 2007 Oct 17<o
<o
3, Chemical force microscopy of single live cells. Nano Lett.</SPAN> 2007 Oct;7(10):3026-30. Epub 2007 Sep 13.<o
<o
4, Combined use of atomic force microscopy, X-ray photoelectron spectroscopy, and secondary ion mass spectrometry for cell surface analysis. Langmuir. 2008 Apr 1;24(7):2955-9. Epub 2008 Feb 1.<o
<o
5, Atomic force microscopy and chemical force microscopy of microbial cells. Nat Protoc. 2008;3(7):1132-8. http://www.nature.com/nprot/journal/v3/n7/abs/nprot.2008.101.html <o
<o
</o
Đây! Bài 3 và 5 em thua, chờ người khác vậy!
http://www.box.net/shared/zpxfycjcyo
Nguyễn Duy Hưng
Senior Member
@Lâm, 2 bài còn lại của a đây
Chemical force microscopy of single live cells
http://ifile.it/nbv5trd
Atomic force microscopy and chemical force microscopy of microbial cells
http://ifile.it/9iy2lct
Chemical force microscopy of single live cells
http://ifile.it/nbv5trd
Atomic force microscopy and chemical force microscopy of microbial cells
http://ifile.it/9iy2lct
Lê Đoàn Thanh Lâm
Senior Member
Cảm ơn Hùng và Hưng rất nhiều.
Nguyễn Thành Đạt
Senior Member
chào các bạn
Bạn có thể cho mình xin quyển sách này đc không:
+ "The protein protocols handbook"
+ second edit
+ edited by: Jond M.Walker
+ humana press
Cảm ơn các bạn trước nhé
Bạn có thể cho mình xin quyển sách này đc không:
+ "The protein protocols handbook"
+ second edit
+ edited by: Jond M.Walker
+ humana press
Cảm ơn các bạn trước nhé
Nguyễn Duy Hưng
Senior Member
Có trên gigpedia.org mà bạn không chịu kiếm nhỉ
http://ifile.it/un6j5o/the_protein_protocols_handbook_0896039404.rar
password: gigapedia.org
http://ifile.it/un6j5o/the_protein_protocols_handbook_0896039404.rar
password: gigapedia.org
Nguyễn Thành Đạt
Senior Member
PS: Hưng cảm ơn bạn, mình cũng đã search rùi nhưng chưa đc. Thứ 2 là theo link của bạn, nhưng mình không biết cách down theo link của bạn, bạn có thể up sang host khác không, thanks
Lê Đoàn Thanh Lâm
Senior Member
Nhờ mọi người lấy giùm mấy bài sau của sciencedirect :<?xml:namespace prefix = o ns = "urn:schemas-microsoft-comfficeffice" /><o></o>
1, Real-time enumeration of adhering microorganisms in a parallel plate flow cell using automated image analysis<o></o>
Journal of Microbiological Methods
Volume 9, Issue 2, February 1989, Pages 73-78
<o></o>
2, Adhesion of Azospirillum brasilense: role of proteins at the cell-support interface<o></o>
Colloids and Surfaces B: Biointerfaces
Volume 7, Issues 3-4, 16 September 1996, Pages 113-128<o></o>
<o></o>
Xin cảm ơn nhiều.
<o></o>
@Đạt : click vào Request download ticket à Download
fficeffice" /><o></o>1, Real-time enumeration of adhering microorganisms in a parallel plate flow cell using automated image analysis<o
></o>Journal of Microbiological Methods
Volume 9, Issue 2, February 1989, Pages 73-78
<o
></o>2, Adhesion of Azospirillum brasilense: role of proteins at the cell-support interface<o
></o>Colloids and Surfaces B: Biointerfaces
Volume 7, Issues 3-4, 16 September 1996, Pages 113-128<o
></o><o
></o>Xin cảm ơn nhiều.
<o
></o>@Đạt : click vào Request download ticket à Download
Nguyễn Phước Hùng
Senior Member
Nhờ mọi người lấy giùm mấy bài sau của sciencedirect :<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com
1, Real-time enumeration of adhering microorganisms in a parallel plate flow cell using automated image analysis<o
Journal of Microbiological Methods
Volume 9, Issue 2, February 1989, Pages 73-78
<o
2, Adhesion of Azospirillum brasilense: role of proteins at the cell-support interface<o
Colloids and Surfaces B: Biointerfaces
Volume 7, Issues 3-4, 16 September 1996, Pages 113-128<o
<o
Xin cảm ơn nhiều.
<o
@Đạt : click vào Request download ticket à Download
Acc của em chỉ load được bài 1!
http://www.box.net/shared/gu82q53drz
Nguyễn Duy Hưng
Senior Member
ifile.it dễ load mà bạn
bạn tìm cái nút Request download ticket, nhấn vào nó, nó sẽ chuyển qua thành Download file, nhấn thêm 1 lần nữa đề download thôi
@Lâm, bài đầu thư viện trg mình ko có (chắc nó cho là đồ cổ, hi hi), mình chỉ load đc bài thứ 2
http://ifile.it/uo0hbj7
bạn tìm cái nút Request download ticket, nhấn vào nó, nó sẽ chuyển qua thành Download file, nhấn thêm 1 lần nữa đề download thôi
@Lâm, bài đầu thư viện trg mình ko có (chắc nó cho là đồ cổ, hi hi), mình chỉ load đc bài thứ 2
http://ifile.it/uo0hbj7
Lê Đoàn Thanh Lâm
Senior Member
Nguyễn Phước Hùng
Senior Member
Hôm qua buồn ngủ quá nên nhầm vị trí chữ " bài" và số 1. Sorry anh.
Lê Đoàn Thanh Lâm
Senior Member
Như Hưng nói, chắc đấy là đồ cổ rồi. Không lấy được thì thôi, cứ để nó trong tủ kính.
<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com
fficeffice" /><o> </o>Thanks.
Nguyễn Duy Hưng
Senior Member
Như Hưng nói, chắc đấy là đồ cổ rồi. Không lấy được thì thôi, cứ để nó trong tủ kính.
<o
Thanks.
Trên thư viện trg mình có bản hard copy, chứ electronic version thì ko có. Bạn có cần ko thì mình lên thư viện lục cho. Đang nghỉ hè nên lười lên trg lắm, hi hi.
