DNA constructs.
Standard procedures for the isolation and manipulation of DNA were used as described in Ausubel et al. (1). The pDLG42-CSL1 plasmid (29) served as a template to amplify the E. coli tryptophanase (tnaA) gene using Phusion High-Fidelity DNA Polymerase (Finnzymes). For E. coli...
làm giúp em câu này với
Cho 0,1 M dung dịch acetic acid (pKa =4,76) và natri acetate, hãy mô tả cách mà bạn sẽ pha 1,0 lít dung dịch đệm acetate 0,1 M (với pH = 4).
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