Trần Phương
Senior Member
chao cac anh chi
em dang lam bai bao cao ve chu de nay nhung khong hieu:
Fetal hemoglobin gene expression analysis in human bone marrow cells. To demonstrate the ability of the NACA technology to measure the expression of a specific gene directly in crude cell lysate, the expression of the human fetal hemoglobin gene was analyzed in primary bone marrow cells treated with hemin (Fig. 5). To validate the approach, the NACA data were compared with quantitative RT±PCR data generated after RNA purification and subsequent specific amplification (see Materials and Methods). As shown in Figure 5A, the
quantitative RT±PCR revealed a 10-fold increase in gHb gene expression in response to in vitro treatment of the bone marrow cells with hemin as compared with the untreated
control cells. Similar results were obtained with the NACA using the indirect (Fig. 5B) or direct modified capture (Fig. 5C) as described in Figures 1B and 4E, respectively. In addition to validating the NACA technology with `real world samples', the agreement between the NACA and quantitative RT±PCR results demonstrates the experimental advantage of the NACA over conventional PCR-based approaches for this type of
application. Notably, the human fetal hemoglobin gene expression was analyzed using the NACA directly from cell lysates without requiring a time-consuming RNA purification
step or an expensive real-time PCR instrument.
bai goc nam o trang: http://nar.oxfordjournals.org/cgi/reprint/31/6/e25
Tai sao ho su dung hemin lam gi, va tai sao su dung Ribosome Protein L19 lam chung noi.
Mong cac anh chi nao biet giup do dum em.
em dang lam bai bao cao ve chu de nay nhung khong hieu:
Fetal hemoglobin gene expression analysis in human bone marrow cells. To demonstrate the ability of the NACA technology to measure the expression of a specific gene directly in crude cell lysate, the expression of the human fetal hemoglobin gene was analyzed in primary bone marrow cells treated with hemin (Fig. 5). To validate the approach, the NACA data were compared with quantitative RT±PCR data generated after RNA purification and subsequent specific amplification (see Materials and Methods). As shown in Figure 5A, the
quantitative RT±PCR revealed a 10-fold increase in gHb gene expression in response to in vitro treatment of the bone marrow cells with hemin as compared with the untreated
control cells. Similar results were obtained with the NACA using the indirect (Fig. 5B) or direct modified capture (Fig. 5C) as described in Figures 1B and 4E, respectively. In addition to validating the NACA technology with `real world samples', the agreement between the NACA and quantitative RT±PCR results demonstrates the experimental advantage of the NACA over conventional PCR-based approaches for this type of
application. Notably, the human fetal hemoglobin gene expression was analyzed using the NACA directly from cell lysates without requiring a time-consuming RNA purification
step or an expensive real-time PCR instrument.
bai goc nam o trang: http://nar.oxfordjournals.org/cgi/reprint/31/6/e25
Tai sao ho su dung hemin lam gi, va tai sao su dung Ribosome Protein L19 lam chung noi.
Mong cac anh chi nao biet giup do dum em.
