Vuongkhang
Junior Member
The wild-type S. ceravisiae strain, BY4742 and the knockout strain, HAP4∆ in a BY4742 strain background were use in this study. The plasmid pAUR-XKXDHXR, which were use for xylulokinase, xylitol dehydrogenase and xylose reductase expression driven by the yeast phosphoglycerate kinase promoter, was digested with BsiWI and chromosomally integrated into the aur1 locus of BY4742 and that of the HAP4∆ mutant to construct the recombinant strains MA-B42 and B42-DHAP4, respectively.
