Google "DNA extraction from Bacillus"
http://www.bio.net/bionet/mm/methods...ly/096531.html
To obtain genomic DNA, 1.5 ml of each overnight culture
was centrifuged at 13,000 g for 3 min. The pellets were resus-pended
in 1 ml of 10 mM Tris HCl, 10 mM EDTA, 100 mM
NaCl, 2% (v/v) SDS, and 400...
Em chào cả nhà, hiện tại Em đang gặp khó khăn trong việc tách DNA plasmid từ Bacillus, Em sử dụng quy trình:
1. Sol I ( 25% sucrose, 50mM EDTA, 50 mM Tris-HCl pH 8.0) and 5mg of lysozyme per ml.
Sử dụng 200ul hào tế bào, sau đó ủ ở 37 độ 10'.
2. Sử dụng sol II, sol III, (25:24:1), (24:1) như...
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